四君子汤对脾虚大鼠尿木糖排泄率及肠黏膜ATP的影响

Effects of Sijunzi Decoction on Urine′s Xylose Excretion Rate and ATP in Mucosa of Spleen Deficiency Rats

目的:观察四君子汤对脾虚大鼠一般状态、尿D-木糖排泄率、小肠黏膜ATP含量的影响。方法:利血平所致脾虚大鼠分为模型对照组和四君子汤组,另设正常对照组。对溴苯胺法测定尿D-木糖排泄率,生物发光法测定小肠黏膜ATP含量,并分析尿木糖排泄率与小肠黏膜ATP含量改变的相关性。结果:与正常对照组比较,造模后大鼠体质量和尿D-木糖排泄率下降(P<0.01);与模型组比较,经四君子汤治疗后尿D-木糖排泄率提高(P<0.05);造模后大鼠小肠黏膜ATP含量与正常对照组比较无明显差异。尿木糖排泄率改变与小肠黏膜ATP含量改变呈正相关。结论:四君子汤能改善脾虚大鼠木糖吸收功能,但对小肠能量代谢指标ATP含量无明显影响;脾虚大鼠尿木糖排泄率降低可伴随小肠黏膜ATP含量降低。

Objective：To observe the effects of Sijunzi Decoction on D-xylose excretion rate and ATP content in the mucosa membranes of small intestines of rats with spleen deficiency. Methods ： Spleen deficiency model rats were made by reserpine injection. D-xylose excretion rate was measured with p-bromoaniline method, and the ATP content of small intestines mucosa was detected with bioluminescence method. The correlation between D-xylose excretion rate and ATP content of mucosa was also analyzed. Results：Rats＇ body weight and D-xylose excretion rate decreased after reserpine injection （P 〈 0. 01, vs control group）, but increased after treated with Sijunzi Decoction（ P 〈 0.05 ,vs model group）. The ATP content of mucosa showed no significant difference between model group and control group. There was obviously positive correlation between the change of urine＇s D-xylose excretion rate and mucosa ATP content. Conclusion： Sijunzi Decoction has the activity of improving xylose absorption in spleen deficiency rats, but no obvious effect on their mucosa ATP content. The reducing of urine＇s D-xylose excretion rate in spleen deficiency rats is accompanied with the decrease of mucosa ATP content.