红芪多糖诱导人肝癌HEP-G2细胞凋亡的作用机制研究

Mechanism of Hedysari Polysaccharide in the Apoptosis of Human Hepatocellular Carcinoma HEP-G2 Cells

目的:研究红芪多糖对人肝癌HEP-G2细胞的增殖抑制与诱导凋亡作用及其可能机制。方法:应用MTT法、显微荧光术和流式细胞术测定红芪多糖对HEP-G2细胞的增殖、细胞周期及凋亡的影响,并应用流式细胞术测定其对Bc1-2和Bax蛋白表达的影响。结果:红芪多糖对HEP-G2细胞的增殖抑制作用具有时间及剂量依赖性,400μg/mL红芪多糖作用72 h后对HEP-G2细胞抑制率达到33.8%;显微荧光术发现作用72 h后,HEP-G2细胞核明显固缩、凝聚,出现浓染致密的颗粒块状荧光;流式细胞术检测显示200、400μg/mL红芪多糖作用72 h,可以观察到G0前期的亚二倍体DNA峰,但对G0/G1期细胞比例和S期细胞比例无明显影响,而G2/M期细胞比例显著升高(P<0.05);Bc1-2蛋白表达水平降低,Bax蛋白表达水平提高。结论:红芪多糖可通过抑制人肝癌HEP-G2细胞的生长增殖、G2/M期阻滞、诱导细胞凋亡、降低bc l-2蛋白、提高Bax蛋白表达等作用机制发挥抗肿瘤作用。

Objective ： To study the effects of Hedysari polysaccharide （HIES） on the proliferation and apoptosis of human hepatocellular carcinoma HEP-G2 cells and its mechanism. Methods：MTT assay ,flow cytometry and fluorescence microscopy were used to the effects of HPS in anti-proliferation, cell cycle arresting and apoptosis induction. Flow cytometry was also used to detect the effects detect of HPS on protein expressions of Bcl-2 and Bax. Results ： HPS could cause apparent inhibition on cell growth, induce GJM phase arrest and apoptosis on HEP-G2 cells. HPS could down-regulate bcl-2 protein expression and up-regulate Bax protein expression. Conclusion： HPS has anti-tumor effects, maybe by the way of inhibiting the cell growth, arresting the G2/M phase,inducing apoptosis, downregulating bcl-2 and up-regulating Bax protein expression.