摘要
应用植物血凝素与寡糖结构相同或相似的糖复合物专一凝集的原理与方法，结合小鼠排卵试验，间接观察了公驼精清及其经ＤＥ－５２离子交换层析和高效液相层析提取分离的活性组分的蛋白组成。结果表明，公驼精清内糖蛋白含有２种以上的寡糖。
In light of the specific agglutination of phytohemagglutinin (PHA) with sugar containing macromolecules the protein composition and bioactive fractions in seminal plasma (S.P.) of bactrian camel were tested, in which fraction L 2 eluted on DEAE cellurose columns induced the ovulation in camel and fraction L 2 2 separated by HPLC elicited superovulation in mice. The results showed that the oligosaccharide of S.P. might be of complex glycoprotein and that its bioactive fractions including the fraction L 2 2 , an inducing ovulation factor, did not reacted with the tested phytohemagglutinin.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第4期355-358,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金
关键词
双峰驼
精清
诱导排卵因子
寡糖
bactrian camel
seminal plasma
inducing ovulation factor
oligosaccharide
