摘要
牛冷冻精液解冻后，在Ｐｅｒｃｏｌ梯度液中４００ｇ离心２４ｍｉｎ，有效地获得了５７．８７％±８．０％的精子回收率；对照组上游法精子回收率仅为１５．８７％±１．９％（Ｐ＜０．０５）。Ｐｅｒｃｏｌ和上游２种方法分离的精子在受精液中８、１８ｈ后的活率，分别为６０％、１０％和７０％、２０％。２种浓度Ｐｅｒｃｏｌ法分离的精子对不同成熟处理的卵母细胞体外受精（ＩＶＦ）卵裂率的影响：精子最终浓度为２×１０６／ｍＬ时，成熟组Ⅰ（ＬＨ＋ＦＳＨ）和成熟组Ⅱ（ＰＭＳＧ＋ｈＣＧ）的卵裂率分别为５６．１９％和５８．１６％；精子最终浓度为５×１０６／ｍＬ时，２组的卵裂率分别为５９．３０％和６２．７７％，卵裂率在精子浓度不同组间及添加不同激素组间的差异不显著。本试验还研究了２种方法处理的精子对不同成熟处理组的卵裂率和囊胚率，用Ｐｅｒｃｏｌ法处理的精子（２×１０６／ｍＬ）在成熟组Ⅰ获得了５７．１６％的卵裂率和２５．５８％的囊胚率，而上游法分别为６１．９５％和１９．０２％，２种方法相差不显著（Ｐ＞０．０５）；在成熟组Ⅱ获得了６２．１８％的卵裂率和２４．７７％的囊胚率，而上游法分别为７１．１８％和２０．９６％，２种方法的卵裂率相差显著（Ｐ＜０．?
The study was designed for 4 experiments:(1) The centrifugation of the semen on the Percoll gradient treatment at 400 g for 24 min was sufficient to obtain optimal recovery of the motile spermatozoa with the recovery rate of Percoll and swim up treatment being 57.87%±8% and 15.87%±1.9%(P<0.05) respectively. (2) The sperm motility that separated by Percoll and swim up methods at 8 and 18 h in Fert TALP and 2×10 6/mL concentration were 60%, 10% and 70%, 20% respectively. (3) Two concentration of spermatozoa separated by Percoll were added to two maturation groups of oocyte, groupⅠ was treated by LH+FSH and groupⅡ by PMSG+hCG with the cleavage rates at 2×10 6/mL and 5×10 6/mL being 56.19%, 58.16%(P>0.05) and 59.3%, 62.77%(P> 0.05 ) respectively. (4) At 2×10 6/mL spermatozoa concentration, by Percoll and swim up methods, the cleavage rate and blastocyst rate in group Ⅰ were 57.16%, 61.95%(P>0.05) and 25.58 %, 19.02% (P>0.05), in groupⅡ were 62.18%, 71.18% and 24.77%, 20.96% respectively. (5) The cell number of blastocyst, by Percoll and swim up methods were 63.79%±11.34% and 67.17 %±14.81%(P>0.05). These data indicated that there were same development ability in the bovine embryos treated by Percoll and swim up separation procedure and that the Percoll method was simpler, faster, and recovered more motile spermatozoa than that of swim up. It is possible to obtain clean semen prepared for IVF using Percoll method.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第4期394-397,共4页
Chinese Journal of Veterinary Science
