摘要
目的探讨在单侧输尿管梗阻(UUO)性肾病肾间质中血清和糖皮质激素诱导蛋白激酶1(serumand glucocorticoid induced kinase 1,SGK1)的表达及血竭素高氯酸盐(Dracohodin Perchlorate,DP)对其的干预作用。方法用单侧输尿管梗阻的方法致大鼠肾间质纤维化,60只SD大鼠随机分为假手术组、模型组、低剂量和高剂量DP治疗组,分别在造模第3、7、14天后处死各组大鼠,采集静脉血检测肾功能(BUN、SCr)变化,肾组织标本用碘酸-席夫(PAS)染色确证发生纤维化病变,免疫组化技术检测肾组织SGK1蛋白的表达及DP干预后的变化。结果与假手术组比较,模型组大鼠第7、14天血清BUN、SCr水平显著升高(P<0.05),肾间质纤维化形成;与模型组比较,仅有DP高剂量治疗组大鼠血清肾功能指标好转(P<0.05),肾间质纤维化好转。模型组大鼠肾间质SGK1蛋白的表达明显高于假手术组(P<0.05),且随着梗阻时间延长其表达呈增强趋势;仅DP高剂量治疗组大鼠的肾间质SGK1蛋白显著低于模型组(P<0.05)。结论在梗阻性肾病肾间质纤维化进程中,SGK1蛋白表达明显上调,而DP对SGK1蛋白的表达有显著的抑制作用,可能对肾间质纤维化病变起到一定延缓作用。
Objective To investigate the dynamic expression of serum and glucocorticoid induced kinase 1 （SGK1） in rat renal interstitium of unilateral ureteral obstruction （UUO） and the effect of Dracohodin Perchlorate （DP） on the expression of SGK1. Methods The model of rat renal interstitial fibrosis was established by unilateral ureteral obstruction. Sixty Sprague-Dawley rats were randomly divided into four groups which were sham operation group, model group, DP treatment groups with low and high dose. Five rats of each group were sacrificed at 3,7 and 14 days after surgery,respectively. The levels of blood urea nitrogen （BUN）and serum creatinine （SCr） were determined. Renal interstitial fibrosis was confirmed by PAS staining and the expression of renal interstitium SGK1 protein was detected by immunohistochemistry assay. Results Compared to rats in sham operation group the levels of BUN and SCr were increased significantly with the development of interstitial fibrosis at 7 and 14 days in rats of model group（P〈0.05）. Compared to rats of model group renal function and interstitial fibrosis were improved significantly at 7 and 14 days in rats of high dose DP treatment group（P〈0.05）. The expression of SGK1 protein in renal interstitium of rats in model group was up regulated and enhanced progressively in time（P〈0.05）. However,the expression of SGK1 protein in renal interstitium of rats in high dose DP treatment group was reduced significantly in contrast to model group（P〈0.05）. Conclusion The expression of renal SGK1 is increased with the progression of UUO, which suggests that SGK1 may play an important role in the development of obstructive nephropathy. Dracohodin Perchlorate probably could prevent renal interstitium fibrosis by inhibiting the expression of SGK1.
出处
《中西医结合研究》
2009年第4期169-172,176,共5页
Research of Integrated Traditional Chinese and Western Medicine
基金
国家自然科学基金项目(No.30600810)
关键词
蛋白激酶类
梗阻性肾病
血竭素高氯酸盐
Protein kinase
Obstructive nephropathy
Dracohodin perchlorate
