咳嗽变异性哮喘患者Th1/Th2相关上游转录因子及细胞因子的表达

Expression of Th1/Th2 related upper stream transcription factor and cytokine in cough variant asthma patients

目的:观察咳嗽变异性哮喘(CVA)患者在基因和蛋白质水平T辅助淋巴细胞(Th)免疫平衡状况。方法:选取26例CVA患者和14例健康自愿者,采用ELISA法测定外周血干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)水平,实时荧光定量PCR法测定外周血单个核细胞(PBMC)中特异性转录因子T-bet、GATA-3表达情况。结果:病例组血IL-4水平为(3.70±0.52)ng/L,高于对照组的(1.59±0.24)ng/L(t=14.310,P<0.01);病例组PBMC中GA-TA-3的表达强度为(0.57±0.06),较对照组的(0.45±0.03)增高(t=7.018,P<0.01);病例组与对照组比较,INF-γ/IL-4及T-bet/GATA-3比值均降低(t分别为-2.208,-3.170,P均<0.05)。结论:CVA患者体内存在Th1/Th2平衡的紊乱,且以Th2亚群亢进为主。

Aim ： To observe the immune balance of auxiliary T lymphocytes （Th） from aspects of gene and protein in patients with cough variant asthma（CVA）. Methods：A total of 26 cases of patients with CVA and 14 cases of healthy volunteers were collected. The levels of cytokines interferon-γ （IFN-γ） and interleukin-4 （IL-4）in peripheral blood were detected by using ELISA methord,and expressions of the speific transeripton factors T-bet and GATA-3 in peripheral blood mononuclear cell（PBMC） were detected by using real-time quantitative PCR. Results：Plasma levels of IL-4 in CVA group was （3, 70 ± 0.52 ） ng/L, which was higher than （1. 59 ± 0.24） ng/L in the control group （t = 14. 310,P 〈 0.01 ）. Expressions of GATA-3 in PBMC in CVA group was （0.57 ± 0.06） ,which was higher than （0.45 ± 0.03） in the control group （t = 7. 018,P 〈 0.01 ）. Ratios of INF-γ/IL-4 and T-bet/GATA-3 in CVA group were significantly lower than those in control group （t = -2. 208, -3. 170,P 〈 0.05）. Conclusion ：The Thl/Th2 balance is disordered in CVA patients,and the Th2 subsets are main hypersplenism.