系统性红斑狼疮患者血清抗核小体抗体和抗核糖体P蛋白抗体检测

Detection of serum anti-nuclearsome antibody and anti-ribosomal P protein antibody in systemic lupus erythematosus

目的:探讨抗核小体抗体及抗核糖体P蛋白抗体(rRNP)检测在SLE诊断中的临床意义。方法:采用德国欧蒙斑点法检测311例SLE患者血清中抗核小体抗体、抗Sm抗体及抗rRNP抗体。结果:SLE患者血清抗核小体抗体及抗rRNP抗体的阳性率分别为34.4%(107/311)、37.9%(118/311),均高于抗Sm抗体的21.3%(66/311)(χ2分别为13.46,20.87,P<0.05),抗核小体抗体、抗rRNP抗体与抗Sm抗体一致阳性率分别5.5%(17/311)和10.0%(31/311),差异有统计学意义(χ2分别为12.1,22,2,P<0.05)。结论:抗核小体抗体及抗rRNP抗体检测可作为抗Sm抗体的重要补充,尤其对Sm抗体阴性的SLE患者,可减少漏诊率。

Aim： To explore the diagnostic value of anti-nucleosome antibody and anti-ribosomal P protein antibody （rRNP） in patients with systemic lupus erythematosus （SLE）. Methods ：A sandwish ELISA was used to measure the serum levels of anti-nuclearsome antibody,anti-Sm antibody and anti-rRNP of 311 SLE patients. Results： The positive rate of anti-nuclearsome antibody and anti-rRNP were 34. 4% （ 107/311 ） and 37. 9% （ 118/311 ） , respectively, higher than 21.3 % （ 66/311 ） of anti-Sm antibody in the 311 SLE patients （χ2 = 13.46,20.87, P 〈 0.05 ）. The concordant positive rate between anti-nuclearsome antibody, anti-rRNA and anti-Sm antibody were 5.5% （17/311 ） , 10.0% （31/311） , respectively,and had significant differences （χ2 = 12. 1,22,2, P 〈 0.05 ）. Conclusion ： Both anti-nuclearsome antibody and anti- rRNP could play major roles in the diagnosis of SLE.