CA_（125）、端粒酶及细胞学联合诊断良恶性腹水的临床研究

Combined diagnosis of benign and malignant ascites by CA_（125）,telomerase and cytology studies

目的：通过联合检测腹水脱落细胞CA125、端粒酶活性及细胞学检查,为临床鉴别诊断良、恶性腹水提供依据。方法：采集52例各种类型腹水患者腹水,用TRAP-PCR-ELISA银染法检测腹水脱落细胞端粒酶活性,放射免疫分析法检测腹水CA125及病理科常规细胞涂片行细胞学检查。结果：联合检测恶性腹水阳性率58.82%,单检细胞学阳性率29.41%,两者相比有显著差异（P＆lt;0.01）。结论：联合检测腹水脱落细胞CA125及端粒酶活性能提高癌性腹水检出率,是腹水良、恶性鉴别及诊断的良好方法。

Objective： To provide evidence for differential diagnosis of benign and malignant ascites through combined detection of exfoliated cells, telomerase activity and cytology examination. Methods： Ascitic fluids from 52 cases of different types of ascites were collected, and telomerase ac- tivity of exfoliated cells in ascitic fluid was detected by TRAP- PCR - ELISA silver stain method, CAI25 of ascitic fluid was detected by radioimmunoas- say, and cytology examination was conducted by roatine cell smear in department of pathology. Resalts： The positive rate of malignaut ascites by com- bined detection was 58.82%, while that of single cytology detection was 29.41%, a very significant difference （ P 〈 0.01 ） between the two. Conclu- sion： Combined detection of CA125 and telomerase activity in exfoliated cells in ascitic fluid can raise thedetection rate of malignant ascites, being a satis- factory method for differentiation and diagnosis of benign and malignant ascites.