摘要
系统研究了酶液用量、激活剂Co2+的浓度及用量、吸收波长、反应时间,反应温度对酶促反应的影响以及固蓝B盐用量和显色时间对显色体系的影响。实验结果表明,在植物酯酶抑制法测蔬菜农药残留时加入Co2+,可以提高检测的灵敏度,降低敌敌畏农药残留的最低检测限。
The paper systematically studies the influence of the dosage of enzyme, the concentration and dosage of activator Co^2+, the absorbed wavelength, the time and temperature of reaction on enzymatic reaction and the influence of the dosage of Fast Blue B Salt and the time of color development on the system of color development. The result shows that adding Co^2+ to the plant esterase to detect the pesticide residues in vegetables and fruits can increase the sensitivity of detection and cut down the lowest detection limit.
出处
《济源职业技术学院学报》
2009年第2期54-56,118,共4页
Journal of Jiyuan Vocational and Technical College
关键词
Co2+
植物酯酶
蔬果
农药残留
Co^2+
plant- esterase
vegetables and fruits
pesticide residues