五味子醇提部位对反复脑缺血再灌注模型小鼠脑能量代谢的影响

Effect of Schisandra Chinensis Baill distilled by ethanol on energy metabolism in brain of repeated cerebral ischemia-reperfusion model mice

目的:探讨五味子醇提部位对反复脑缺血再灌注模型小鼠脑能量代谢的影响。方法:将动物随机分成6组,分别灌服尼莫地平片混悬液、银杏叶混悬液及大、小剂量五味子醇提部位混悬液,假手术组(空白组)和模型组给予同体积生理盐水,每日1次,连续给药15d,采用双侧颈总动脉夹闭,建立反复脑缺血再灌注致学习记忆障碍小鼠模型。术后48h后,断头取脑,取一半脑称质量,加9倍生理盐水制成10%脑匀浆,测乳酸含量,乳酸脱氢酶、乙酰胆碱酯酶和ATP酶活力。结果:与模型组比,小剂量五味子醇提部位可明显降低模型小鼠乳酸含量,大剂量五味子醇提部位可显著提高模型小鼠乳酸脱氢酶活力;大、小剂量五味子醇提部位组可显著提高模型小鼠乙酰胆碱酯酶活力,大剂量五味子醇提部位组能显著提高模型小鼠Na+-K+-ATP、Ca2+-ATP酶活力。结论:五味子醇提部位可显著改善反复脑缺血再灌注模型小鼠脑能量代谢。

Objective：To observe the effect of Schisandra Chinensis Baill distilled by ethanol on energy metabolism in brain of repeated cerebral ischemia-reperfusion model mice, Method：The mice were randomly divided into six groups, respectively irrigating the groups of Nimodipine suspension, ginkgo leaf suspension, and large and small doses of Schisandra Chinensis Baill distilled by ethanol, sham group （blank） and the model group for the same volume of saline, once a day, 15 days for delivery. Model mice of cerebral ischemia-reperfusion injury was established by bilateral common carotid artery blocking. 48 hours after operation, the rats were decapitated, weight half of the brain and put 9 times saline into it, then the level of LD, vitality of LDH, AchE and ATP were measured. Results：Compared with model group, small doses of Schisandra Chinensis Baill distilled by ethanol could markedly reduce the content of LD of mice of repeated cerebra/ischemia-reperfusion, large doses of Schisandra Chinensis Baill distilled by ethanol could notably improve the vitality of LDH; large and small doses of Schisandra Chinensis Balll distilled by ethanol could both notably improve the vitality of AchE, large doses of Schisandra Chinensis Balll distilled by ethanol could notably improve the vitality of Na^＋-K^＋-ATP, Ca^2＋-ATP. Conclusion： Schisandra Chinensis Baill distilled by ethanol could markedly improve the energy metabolism of the model of repeated cerebral ischemia-reperfusion of mice.