沙漠嘎子中总黄酮及圣草酚-7-甲醚、异樱花素的含量测定

Determination of total flavonoids,eriodictyol-7-methylether and isosakuranetin in seeds of Artemisia halodendron Turcz

目的:建立沙漠嘎子中总黄酮及圣草酚-7-甲醚、异樱花素的含量测定方法。方法:以圣草酚-7-甲醚为指标性成分,用紫外分光光度法(UV)测定总黄酮的含量,检测波长506nm;采用高效液相色谱法(HPLC),使用YMC-Pack ODS-A(5μm,4.6mm×250mm)色谱柱,以乙腈-0.2%磷酸溶液(35∶65)为流动相,流速1.0ml/min,检测波长288nm,以外标法计算沙漠嘎子中圣草酚-7-甲醚和异樱花素的含量。结果:总黄酮量在0.1228-0.7368mg范围内线性关系良好(r=0.9991),平均回收率(n=6)为100.5%(RSD=1.41%);圣草酚-7-甲醚、异樱花素进样浓度分别在3.016-30.16μg/ml(r=0.9999),3.304-33.04μg/ml(r=0.9999)范围内呈良好的线性关系,平均回收率(n=6)为98.9%(RSD=1.20%),96.8%(RSD=1.38%)。结论:紫外分光光度法和高效液相色谱法均简便、准确、重现性好,适用于沙漠嘎子中总黄酮及圣草酚-7-甲醚、异樱花素的含量测定。

Objective： To establish a method for determination of total flavonoids, eriodictyol-7-methylether and isosakuranetin in Artemisia halodendron seeds. Method： The content of total flavonoids was determined by ultraviolet spectrophotometry at 506 nm, and compared with eriodictyol-7-methylether as a reference substance; the content of eriodictyol-7-methylether and isosakuranetin was determined by RP-HPLC, the analytical column was YMC-Pack ODS-A column （5μm,4.6 mm×250 mm）, the mobile phase consisted of acetonitrile-0.2% phosphoric acid aqueous （35：65） at a flow rate of 1.0 ml·min^-1, and the detection wavelength was 288 nm. Results： The linear range of total flavonoids was 0.1228-0.7368mg （r=0.9991） and the average recovery （n=6） was 100.5% （RSD=1.41%）; the linear ranges of eriodictyol-7-methylether and isosakttranetin were 3.016-30.16μg/ml （ r=0.9999 ） and 3.304-33.04μg/ml （ r=0.9999 ） , respectively; the average recoveries （n=6） were 98.9% （ RSD=1.20% ） and 96.8% （ RSD=1.38% ） , respectively. Conclusion： The methods of UV and HPLC are simple, accurate with good reproducibility and suitable for determination of total flavonoids, eriodictyol-7-methylether and isosakuranetin.