滤除白细胞与MB-P法病毒灭活对血浆生化指标的影响

The impact of methylene blue plus light method for virus inactivation and leukocyte proof filter on plasma biochemistry indexes

目的探讨应用滤除白细胞及MB-P病毒灭活技术对血浆生化指标的影响。方法随机采集无偿献血合格者血液标本20份,400ml/份。将每份新鲜血浆在制备过程中平均分为试验组与对照组。所有标本同时进行相关生化指标检测。结果试验组经滤除白细胞后FRUC浓度、FHb及CO2增高明显,与对照组比较有显著性差异(P<0.01)。试验组经MB-P法病毒灭活后FRUC与P3+浓度明显增高,FHb下降较快,与对照组及病毒灭活后比较均有显著性差异(P<0.01)。其它生化指标变化不大。结论滤除白细胞及病毒灭活后的血浆制作,对血液生化指标影响均较小或轻,适用于临床输用血(浆)需求。

Objective To evaluate the impact of methylene blue plus light method for virus inactivation and leukocyte proof filter on plasma biochemistry indexes. Method Twenty blood sample were randomly taken out, 400ml/share, dimidiated to trail group and control group. Biochemistry indexes of all the samples were analyzed at the same time. Result The levels of FRUC, CO2, FHb changed significantly （P〈0.01） after filtration, whereas the levels of FRUC, p3~, FHb changed significantly （P〈0.01） after methylene blue plus light. Conclusion The impact of methylene blue plus light method for virus inactivation and leukocyte proof filter on plasma biochemistry indexes are less;it is applicable for clinical blood transfusion.