β-七叶皂苷钠对大鼠脑出血后血清TNF-α含量的影响

Effect of β-sodium aescinate on content of TNF-α in serum of experimental rats with cerebral hemorrhage

目的探讨β-七叶皂苷钠对大鼠脑出血后TNF-α及脑水肿的影响。方法采用Rosenberg脑内立体定向注射Ⅶ型胶原酶诱发大鼠脑出血模型。将SD大鼠78只随机分为假手术组、出血组、出血干预组及对照组。以干湿重法测定脑含水量,用ELASA法测量血清TNF-α含量。利用草酸-焦锑酸钾电镜化学技术观察脑出血周围组织超微结构的改变。结果治疗组脑含水量明显低于对照组(P<0.01)。出血后12h TNF-α升高达高峰,此后逐渐下降,β-七叶皂苷钠治疗组在出血后24h及3d较出血组及对照组明显减少,7d则与后两组无明显差异。超微结构观察治疗组神经元及内皮细胞肿胀程度较对照组明显减轻。结论β-七叶皂苷钠能明显减少脑出血模型大鼠血清TNF-α水平以及减轻脑水肿。

Objective To investigate the effect of β-sodium aescinate on TNF-αand brain edema in rats with cerebral hemorrhage. Methods Seventy-eight adults SD rats were randomly divided into sham operration group,hemorrhage group, intervention group treated with β-sodium aescinate and control group.Animal model was established by injecting collegenase Ⅶ into left candoputamen of adult rat under stereotaxic apparatus. The cerebral water content was measured by dry-wet weight method.To mesure the TNF-α content in serum with ELASA . The ultrastructure histopathologic changes of peri lesion sons of cerebral hemorrhage were observed under electronic microscope. Results The cerebral water content in the intervention group were significently different from those in the control group. （P〈0.01） The TNF-α content of therapeutic group： treated with β-sodium aescinate decrease significantly at 24h and 3day after hemorrhage.However,There is no difference between there groups at 7 day after hemorrhage.The histopathologic results showed that the edema of neuron and endothelial cell were more severe in control group than that of intervention group. Conclusion β-sodium aescinate is effective for treatment of cerebral hemorrhage and cerebral edema.