摘要
目的：建立高效液相色谱测定草珊瑚中富马酸含量的方法。方法：采用高效液相色谱法，固定相为Dia-monsilC180-（5um，250×4．6mm），以乙腈一水一磷酸（2：98：0．05）作为流动相，检测波长为208nm。结果：富马酸在54．35-434．8”g范围内线性关系良好，r2—0．9999，平均回收率为99．95％，RSD-1．33％。结论：该方法操作简单、准确，重复性好，可适用于草珊瑚药材的质量控制。
Objective.. To establish a HPLC method for the quantitative determination of fumaric acid in Sarcandra glabra （Thunb.） Nakai. Methods：The determination was carried out with Diamonsil C18 column （ 5μm, 250× 4. 6mm）, using acetoni- trile-water-H3 PO4 （2 ： 98 ：0. 05, V/V） as mobile phase with isocratic elution, at a flow rate of lmL/min and detected at the wave- length 208nm. Results： Fumaric acid had a good linear relationship between 54. 35~434. 8μg （r2 = 0. 9999）,and the average re- covery rate of fumaric acid is 99.95%, RSD is 1.33%. Conclusion.. The method is very simple and accurate, and has good reap- pearance. It can be used for the quality control of Sarcandra glabra （Thunb.） Nakai.
出处
《亚太传统医药》
2009年第8期43-46,共4页
Asia-Pacific Traditional Medicine
关键词
高效液相色谱
草珊瑚
富马酸
含量测定
HPLC
Sarcandra glabra （Thunb.） Nakai
Fumaric acid
Quantitative determination
