AcSDKP对正常人真皮成纤维细胞表达TGF-β1的影响

Effects of AcSDKP on TGF-β1 Expression in Human Dermal Fibroblasts

目的探讨N-乙酰基-丝氨酰-天冬氨酰-赖氨酰-脯氨酸(AcSDKP)对真皮成纤维细胞表达TGF-β1的影响。方法对人皮肤成纤维细胞原代培养及鉴定后,给予不同浓度AcSDKP(10-7mol/L、10-8mol/L、10-9mol/L、10-10mol/L、10-11mol/L)干预,设立空白对照组。WST-8法检测人皮肤成纤维细胞增殖;RT-PCR技术检测人皮肤成纤维细胞TGF-β1mRNA表达;酶联免疫法检测培养细胞上清液TGF-β1含量。结果与空白对照组相比,各浓度组对成纤维细胞的增殖都有抑制作用,其中10-10mol/LAcSDKP抑制成纤维细胞增殖作用最强;10-7mol/L、10-8mol/L、10-9mol/L、10-10mol/LAcSDKP对TGF-β1mRNA表达有显著的抑制作用,且各浓度组间的抑制作用无差异;10-8mol/L、10-9mol/L、10-10mol/LAcSDKP可使培养上清液中的TGF-β1显著减少,各浓度组间差异无显著性。结论AcSDKP能够抑制人皮肤成纤维细胞增殖及TGF-β1的生成,有望成为病理性瘢痕防治的新方法。

Objective To study the effects of N-acetyl-seryl-aspartyl-lysyl-praline （AcSDKP） on TGF-β1 synthesis in human dermal fibroblasts. Methods Human dermal fibroblast after Primary cultures and identification were subjected to AcSDKP （10^-7 mol/L, 10^-8 mol/L, 10-9 mol/L, 10^-10 mol/L, 10^-11 mol/L, respectively） treatment. The control group was not seeded AcSDKP. The proliferation of human dermal fibroblasts were detected by WST-8. The expression of TGF-β1 mRNA were analyzed with RT-PCR. TGF-β1 in the supernatant were determined by enzyme-linked immunosorbent assay. Results Compared with control group, the proliferation of human dermal fibroblasts were inhibited by all AcSDKP groups, and 10^-10 mol/L AcSDKP had the most effects; 10^-7 mol/L, 10^-8 mol/L, 10^-9 mol/L, 10^-10 mol/L AcSDKP significantly inhibited the expression of TGF-β1 mRNA, and the different groups had no different effects; Expression of TGF-β1 in the supernatant were significantly inhibited by 10^-8 mol/L, 10-9 mol/L, 10^-10 mol/L AcSDKP. Conclusion The proliferation and expression of TGF-β1 in human dermal fibroblasts were significantly inhibited by AcSDKP. AcSDKP has the best preventive effect on the pathological scar.