CK-19在甲状腺乳头状癌及甲状腺乳头状增生患者外周血中的表达

Expression of CK-19 in peripheral blood of patients with papillary thyroid carcinoma and papillary thyroid hyperplasia

目的检测细胞角蛋白(CK-19)在甲状腺乳头状癌(PTC)和结节性甲状腺肿并乳头状增生患者血清中的表达,筛选甲状腺乳头状癌的辅助诊断指标。方法采用酶联免疫吸附试验(ELISA)法检测结节性甲状腺肿并乳头状增生25例、乳头状癌25例及正常人25例血清中CK-19的表达。结果CK-19表达水平以甲状腺乳头状癌高,结节性甲状腺肿伴乳头状增生居中,正常人低,三者差异有显著性(F=8.371,P<0.05);CK-19表达水平在甲状腺乳头状癌伴淋巴结转移比无伴淋巴结转移者更高,两者差异有显著性(t=5.371,P<0.05)。结论甲状腺乳头状癌患者外周血中CK-19表达率增高;CK-19对于甲状腺乳头状癌伴淋巴结转移的诊断具有一定的指导作用。

Objective To detect serum cytokeratin（ CK - 19） in patients with papillary thyroid carcinoma（PTC） and nodular goiter with papillary hyperplasia, in order to suggest a diagnostic indicator for thyroid papillary carcinoma. Methods CK - 19 expression was determined in 25 cases of nodular goiter with papillary hyperplasia, 25 cases of papillary carcinoma, and 25 healthy subjects with enzyme - linked immunosorbent assay（ ELISA）. Results CK - 19 expression level was high in papillary thyroid carcinoma, middle in nodular goiter with papillary hyperplasia, and low in healthy subjects（ F = 8. 371, P 〈 0.05 ）. CK - 19 expression level in papillary thyroid carcinoma with lymph node metastasis was higher than that in papillary thyroid carcinoma without lymph node metastasis （ t = 5. 371, P 〈 0.05 ）. Conclusion CK - 19 expression is increased in patients with papillary thyroid carcinoma. CK - 19 plays a guiding role in the diagnosis of papillary thyroid carcinoma with lymph node metastasis.