摘要
在无籽西瓜离体培养中 ,培养基中较高的氨态氮 /硝态氮比例、 BA浓度过高、琼脂和蔗糖浓度过低以及高温、弱光的培养条件均可导致玻璃化苗的增加。在培养基中添加青霉素 ( 60mg· L-1)和氯化胆碱 ( 30 0 mg· L-1)均可有效的抑制玻璃化苗的发生。
The experiment results showed that the vitrification was caused by higher NH+4N/NO- 3 N ratio and concentration of BA,lower concentration of agar and sugar in the media,and highter temperature and lower light intensity culture condition.It was also found that vitrification was effectively inhibited by supplemented with penicillin (6 0 mg·L- 1 ) and choline chloride(30 0 mg·L- 1 ) in the media.
基金
广西区教育厅资助的科研项目
