子宫内膜息肉中Bcl-2、Bax和Caspase3表达及其意义

Expression levels and significance of Bcl-2,Bax and Caspase 3 in women with endometrial polyps.

目的:检测子宫内膜息肉中Bcl-2、Bax和Caspase3表达,探讨其在子宫内膜息肉发生、发展中的作用。方法:标本选自2004年1月～2006年3月浙江大学医学院附属妇产科医院住院患者。子宫内膜息肉63例为研究组,正常子宫内膜55例为对照组,采用免疫组织化学二步法(Envision system)检测Bcl-2、Bax和Caspase3的表达。结果:无论增生期还是分泌期,研究组Bcl-2表达均显著高于对照组(P<0.05);而增生期Bax的表达两组差异无显著性意义(P>0.05),分泌期Bax的表达研究组显著低于对照组(P<0.05)。增生期研究组和对照组中Caspase3均有一定程度的表达,但两组相比无显著性差异(P>0.05),分泌期研究组和对照组中Caspase3的表达均增强,但研究组中Caspase3的表达显著低于对照组(P<0.05)。结论:增生期Bcl-2的高表达及分泌期Bax的低表达可能参与子宫内膜息肉的形成。分泌期子宫内膜息肉中Caspase3的表达增高,但明显少于正常子宫内膜,提示Caspase3可能参与子宫内膜息肉的发生、发展。

Objective： To detect the expression levels of Bcl -2, Bax and Caspase 3 in endometrial polyps, explore the effect of Bcl - 2, Bax and Caspase 3 on the pathogenesis and progress of endometrial polyps. Methods ： The expression levels of Bcl - 2, Bax and Caspase 3 in endometrial polyps samples from 63 cases with endometrial polyps （study group） and 55 cases with normal endometrium （control group） were detected by immunohistochemistry from January 2004 to March 2006. Results： In proliferative phase and secretory phase, the levels of Be1-2 in study group were significantly higher than those in control group （P 〈0. 05） . In proliferative phase, there was no significant difference in the level of Bax between study group and control group （ P 〉 0. 05 ）, while in secretory phase, the level of Bax in study group was significantly lower than that in control group （ P 〈 0. 05 ） . In proliferative phase, there was no significant difference in the level of Caspase 3 between study group and control group （ P 〉 O. 05 ） , while in secretory phase, the level of Caspase 3 in study group was significantly lower than that in control group （ P 〈 0. 05 ） . Conclusion： Upregulated Bcl - 2 level and downregulated Bax level may participate in the pathogenesis of endometrial polyps. In secretory phase, the level of Caspase 3 increases, which is significantly lower than that in normal endometrium, indicating a role of Caspase 3 playing in the pathogenesis and progress of endometrial polyps.