摘要
目的探讨从余甘子叶提取分离得到的活性成分没食子酸的抗肿瘤作用。方法采用MTT法和细胞集落形成法观察没食子酸对人肝癌细胞株Bele7404、人胃癌细胞株SGC7901、小鼠肝癌细胞株H22和小鼠肉瘤细胞株S1804种肿瘤细胞的体外抑制作用。结果MTT法和细胞集落形成法测定的结果均显示,当没食子酸浓度在2.5μg/m l以上时,4种肿瘤细胞株的生长均受到明显抑制。结论余甘子叶提取成分没食子酸在体外对不同组织来源的肿瘤细胞增殖有明显的抑制作用。
Objective To isolate the anti - tumor activity components for gallic acid from the ethanol extract of the ethyl acetate fractions of the leaves of Phyllanthus emblica L. Methods The growth inhibition was analyzed by MTT and cell colony measurement technique in the hepatocellular carcinoma Bele7404, stomach carcinoma SGC7901, hepatoma H22 and fibrosarcoma S180 cells line in vitro. Results MTT assay and cell colony measurement technique were indicated, the growth of four tumor cells were obviously inhibited when gallic acid concentration was at 2.5 μg / ml and above. Conclusion The results suggest that gallic acid is cytotoxic to the variety of the cancer cells.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第8期1954-1955,共2页
Lishizhen Medicine and Materia Medica Research
基金
广西科技学技术厅项目(No.0639051No.0718002-2-1)
广西高校人才小高地建设创新团队资助计划
关键词
余甘子叶提取物
没食子酸
MTT法
细胞集落形成法
体外抗肿瘤作用
Extract from leaves of Phyllanthus emblica L.
Gallic acid
MTT
Cell colony measurement technique
Anti - cancer effect in vitro
