摘要
采用化学修饰法研究了史氏芽胞杆菌Bacillus smithiiT7产耐热菊粉酶活性中心氨基酸残基,发现该酶活性中心存在一个组氨酸残基和一个谷氨酸(或天冬氨酸)残基.修饰前后的酶动力学参数变化表明组氨酸残基参与了底物的结合和催化过程,而谷氨酸(或天冬氨酸)的羧基亲核攻击促使底物分解.邹氏作图法证明酶活性中心存在两个必需的色氨酸残基,荧光和圆二色光谱研究表明色氨酸残基在酶的催化和酶的耐热性方面起重要作用.
The amino acid residues participating in the active site of the extracellular thermostable endo-inulinase from Bacillus smithii T7 were investigated by chemical modification. The results show that there are one histidine residue and one carboxylate residue in the active site. The kinetic study of the enzyme before and after modification by diethylpyrocarbonate and carbodiimide showed that one histidine residue might play a key role in substrate binding and catalysis, and one carboxylate residue located in the active site may act as a nucleophile base for the cleavage of substrate. The Tsou's plot analysis indicated that the inactivation of the enzyme is dependent upon the modification of two essential tryptophan residues. Fluorescence and circular dichroism spectroscopy assays and the thermal inactivation study of the modified enzyme also confirmed that tryptophan residues play an important role in the catalysis and thermal stability of the inulinase.
出处
《催化学报》
SCIE
CAS
CSCD
北大核心
2009年第7期673-678,共6页
基金
教育部留学回国人员科研启动基金([2006]331)
国家重点基础研究发展计划(973计划
2009CB724706)
关键词
菊粉酶
化学修饰
活性中心
氨基酸残基
催化
inulinase
chemical modification
active site
amino acid residue
catalysis