微血管计数与乳腺癌血行转移及预后相关性研究

Microvessel Count in Breast Cancer in Relation to Hematogenous Metastasis and Prognosis

目的：为探讨乳腺癌中肿瘤血管生成与血行转移及预后的相关性，对３０例原发浸润性乳腺癌腋淋巴结阴性者进行回顾性研究（其中１５例出现血行转移）。方法：肿瘤标本常规处理后ＦⅧ因子及生物素标记的荆豆素（ＵＥＡ）免疫组化染色，光学显微镜下计数２００倍视野微血管数。结果：出现血行转移者平均微血管数１０８个，未出现血行转移者平均微血管数４５个（Ｐ＜０．００１）。微血管数＜８０个者其总生存率（ＯＳ）及无瘤生存率（ＲＦＳ）分别为８７．５％、８１．２５％，而≥８０个者其ＯＳ及ＲＦＳ分别为２８．６％、１４．２８％（Ｐ＜０．００２）。结论：瘤内微血管计数可作为判断乳腺癌预后的指标。

Objective: To study how tumor angiogenesis correlates with the metastasis and prognosis of breast cancer. Methods: The microvessel count in 30 node negative primary breast cancers (15 with hematogenous metastasis, 15 without) were studied. Intratumoral microvessels were identified by immunostaining in formalin fixed, paraffin enbedded sections with factor VIII and Ulex Europaens I Agglutinin (UEA) under optical microscope. Results:The mean number of microvessels found in the patients with hematogenous metastasis was 108, while in patients without metastasis was 45 ( P <0.001). The overall survival (OS) and recurrence free survival (RFS) rates of patients with the microvessel number less than 80 were 87.5% and 81.25% respectively, whereas with the number greater than or equal to 80, the rates of OS and RFS were 28.6% and 14.28% ( P <0.002) respectively.Conclusion: The microvessel count in the areas of most intensive neovascularization in node negative invasive breast cancer may be an independent predictor of prognosis.