草鱼胆汁酸与细菌生长试验

STUDY ON BILE ACID FROM GRASS CARP BILE AND ITS BACTERIOSTATIC EFFECT

本文研究了草鱼结合胆汁酸盐及游离胆汁酸盐的抑菌作用。研究结果表明，草鱼结合胆汁酸盐和游离胆汁酸盐对三种革兰氏阳性菌，如金黄色葡萄球菌（Staphylococcusaureus）、藤黄八叠球菌（Sarcinalutea）和枯草芽孢杆菌（Bacillussubtilis）有抑菌作用，能够明显地抑制革兰氏阳性菌的生长。草鱼结合胆汁酸盐像乳糖培养基中的牛胆盐一样，能够抑制革兰氏阳性菌的生长，而有利于革兰氏阴性菌如大肠杆菌（Bacilluscoli）的生长。草鱼游离胆汁酸盐像GN增菌液中的去氧胆酸钠一样，能够抑制革兰氏阳性菌的生长，而对革兰氏阴性菌痢疾志贺氏菌（Shigelladysenteriae）的生长则无影响。

The bacteriostatic effect of both free and combined bile salts from grass carp bile was studied in this paper. The experimental results show that both salts remarkably exerted their bacteriostasis to three species of such Gram positive bacteria as Staphylococus aureus,Sarcina lutea and Bacillus subtilis. The combined salt was quite similar to cow bile salt cultured in lactose bile medium, which showed bacteriostasis to Gram positive bacteria, but not to Gram negative ones like Bacillus coli. As to the free bile salt, it was similar to deoxycholic acid in GN-enriched medium and showed bacteriostasis to Gram positive bacteria, but no effect on the growth of Shigella dysenteriae.