液体悬浮培养促进铁皮石斛原球茎高效诱导、增殖的研究

Study on the Induction and Proliferation of Protocorm in Dendrobium officinale by Suspension Culture

用正交设计方法对铁皮石斛原球茎具有高效增殖影响的激素(BA,NAA,KT,马铃薯汁)以及配比进行研究,结果表明:以茎段为外植体在培养基MS+BA2.0 mg/L+NAA2.0 mg/L+马铃薯汁10%+糖3%,具有很好的原球茎诱导作用,50 d后诱导率达95.20%;原球茎在1/2MS+BA2.0 mg/L+NAA1.0 mg/L+KT0.5 mg/L+糖3%的培养基上,以液体悬浮培养,原球茎增殖达49.032 g/50d。

To study on the effects of plant growth regulator （ BA, NAA, KT, and Potato juice） on the induction and proliferation of Dendrobium officinale protocorm by orthogonal design. The results showed that the optimum induction medium is MS ＋ BA2.0 mg/L ＋ NAA2.0 mg/L ＋ potato juice10% ＋ sugar 3%, the induction rate of protocorm is 95.20% after 50 days; the plants are transplanted in the proliferation medium of 1/2MS ＋ BA2.0 mg/L ＋ NAA1.0 mg/L ＋ KT0.5 mg/L ＋ sugar 3% after the protocorm are inducted. Proliferation rate of Dendrobium officinale protocorm in liquid medium is 49. 032 g/50d.