健康人群真胰岛素水平研究

Study on true insulin levels in healthy population

目的建立健康人群的真胰岛素(TI)正常参考范围,以助于研究因胰岛素改变而引起的内分泌疾病。方法健康人600例,其中男300例为男性组,女300例为女性组,年龄16～40岁为低年龄组,年龄40～60岁为高年龄组。于上午08:00空腹取静脉血3mL。采用放射免疫分析法(RIA)和化学发光法测定胰岛素。结果健康人组RIA法胰岛素为(121.18±39.5)pmol/L,化学发光法TI为(33.00±19.06)pmol/L比较两种方法差异有统计学意义(P<0.01)。男性组TI为(34.22±20.89)pmol/L,女性组TI为(32.12±16.84)pmol/L性别组比较差异无统计学意义(P>0.05)。低年龄组TI为(32.15±18.68)pmol/L,高年龄组TI为(33.32±18.67)pmol/L。年龄组比较差异无统计学意义(P>0.05)。结论健康男性TI正常参考值范围为(9.08～95.34)pmol/L;健康女性TI正常参考值范围为(12.35～92.78)pmol/L。TI测定能更准确分析胰岛β细胞功能。

Objective To establish true insulin （TI） normal reference range in healthy crowd for helping to study the changes in insulin induced endocrinic diseases. Methods 600 cases of healthy controls,of which 300 males as the male group,300 women as the female group,aged 16-40 years old as the low age group,age 40-60 years as high old age group. At 8：00 3 mL fasting venous blood was collected. Using radioimmunoassay （RIA） and chemiluminescence method for determination of insulin. Results Insulin level in normal group for insulin RIA method was （121.18± 39.5） pmol/L, for the TI chemiluminescence （33.00 4± 19.06） pmol/L, there was a statistical difference （P〈0.01）. TI for the male group was （34. 224±20.89） pmol / L,for female group （32.12 4± 16.84） pmol/L with no significant difference in sexes （P〉0.05）. TI for the low age group was （32.15 4± 18.68） pmol/L,for the highage group, TI was （33. 32 4± 18. 67） pmol/L with no siatistical difference in age （P〉 0.05）. Conclusion Healthy male TI range of normal reference values is （9.08-95.34） pmol/L; the healthy women TI range of normal reference values is （12.35-92.78） pmol/L. TI determination can analyse islet β-cell function more accurately.