外源性碱性成纤维细胞生长因子对放射后大鼠学习记忆能力的保护作用

Therapeutic effects of exogenous bFGF on learning and memory ability of post-radiation rats

目的探讨外源性碱性成纤维细胞生长因子（bFGF）对放射后大鼠学习记忆能力的影响。方法利用分割照射建立的迟发性放射性脑损伤（RIB）模型鼠24只，采用尾静脉直接注射给药法给予外源性bFGF，3个月后进行学习记忆能力检测，同时观察脑组织病理变化及FGF受体1（FGFR1）表达情况。结果bFGF组平均寻台潜伏期[（56．57±4．29）s]和第一象限路径与总路径长度百分比[（21．57±1．47）％]均较模型组[（118．24±10．79）s，（6．95±0．75）％]有显著改善（P〈0．01）；HE染色海马区域完整细胞计数bFGF组[（115．61±12．82）个]较模型组[（70．86±8．51）个]明显增多（P〈0．01）；IHC结果显示，bFGF治疗组海马内FGFR1表达水平（31．50±1．59）较模型组（12．03±1．68）明显增高（P〈0．01）。结论外源性bFGF可通过诱导FGFR1表达对RIB模型鼠学习记忆能力起到一定的保护作用。

Objective To explore the therapeutic effects of basic fibroblast growth factor （bFGF） on learning and memory ability of post-radiation rats. Methods 24 Sprague-Dawley rats were treated with 30 Gy fractionated irradiation and then randomly divided into 2 groups. Exogenous bFGF and normal sodium were administered intravenously through caudal vein after modeling. Morris maze tests were taken to study the learning and memory ability of rats 3 months after injection. The pathologic feature was studied by using H＆E staining and IHC staining of FGF receptor 1 （ FGFRI ）. Results Statistie differences of average escape lateney and search strategy were found belween model group [（ 118.24±10.79） s, （ 6.95±0.75 ） % ] and bFGF group [（ 56.57±4.29） s, （21.57±1.47）% , P〈0.01]. Intact cell number in hippoeampus was more in bFGF group （115.61±12.82） than model group （70.86±8.51 , P 〈 0.01）. There was weak expression of FGFR1 in hippocampus of model group（12.03±1.68）,which were fewer than bFGF group （31.50±1.59, P〈0.01）. Conclusion Exogenous bFGF injected intravenously through caudal vein has therapeutic effcets on learning and memory ability of post-radiation rats by inducing the expression of FGFR1.