摘要
目的:建立反相高效液相色谱-二极管阵列检测法(HPLC-DAD)测定动物性食品中维生素E(α-生育酚)的含量。方法:称取一定量鹅肉样品加入乙醇、氢氧化钾及抗坏血酸,100℃水浴皂化30min,乙醚提取后,减压浓缩,氮气吹致干,用脱醛乙醇溶解,进样10μL分析;色谱条件为:SunFireTmC18柱C18柱(15cm×4.6mm,5μm),流动相:甲醇∶水(98∶2),柱温30℃,检测波长300nm。结果:维生素E在0.10-50.0mg/L浓度范围内相关系数r=0.9985。结论:该方法能快速、灵敏、准确实现对动物性食品中脂溶性维生素E的含量分析。
Object： To determinate the concentration of a -tocopherol in the goose using HPLC with DAD. Methods： Goose was added in anhydrous alcohol, potassium hydroxide and vitamin C. The mixture was shaken and saponified at 100℃ for 30 minutes, following extracted by ether and dried by evaporation, dissolved in ethanolgrain alcohol, and analyzed by HPLC-DAD, α -tocopherol was separated on SunFire TmC18, 15 cm×4.6 mm, 5μm column and detected at 300nm by using methanol：water （98：2） as mobile phase when the column temperature was 30℃. Result： The linear ranges of determination of a -tocopherol was 0.1-50.0mg/L（r=0.9985）. The α-tocopherol in goose is 0.264μg/mL. Conclusion. The method is sensitive, simply and accurate. It can be used to determine the concentration of α-tocopherol in animal food.
出处
《肉类研究》
2009年第8期59-61,共3页
Meat Research
