摘要
分别采用4-AP致小鼠过敏性皮肤瘙痒模型和组胺致小鼠足肿胀试验，探索地肤子总皂苷硅胶柱层析的活性部位和量效关系。结果表明，地肤子抗过敏活性成分主要集中在氯仿一甲醇为9：1的2、3BV，8：2的1—3BV，7：3的1—3BV洗脱液中；地肤子总皂苷抗过敏作用的有效阈剂量为32mg／kg，良效剂量为150mg／kg，且抗过敏性瘙痒作用比抗过敏介质的致炎作用强。
The active fractions and the dose - effect relationship of Fructus Kochia Scoparia were studied by using the experimental model of the mice allergic cutaneous pruritus caused by 4 - AP and the test of mice foot swelling caused by histamine. The results showed that the anti -allergic active components mainly existed in the2 and 3 BV eluante of 9 ： 1 CH3Cl-MeOH, 1 -3 BV eluante of 8 ： 2 and 7 ： 3 CH3Cl- MeOH. The effective threshold dose of total saponin of Fructus Kochia Scoparia was 32 mg/kg and the moder- ate effective dose was 150 mg/kg. The results also showed that its resistance to the allergic pruritus was stronger than that to the infammafion - causing effect of anti - allergic medium.
出处
《山东农业科学》
2009年第8期49-52,共4页
Shandong Agricultural Sciences
基金
国家"十一五"科技支撑计划(2006BAD06B01)
教育部高校博士点基金(20060307024)项目资助
关键词
地肤子
总皂苷
抗过敏作用
活性部位
量效关系
Fructus Kochia Scoparia
Total saponin
Anti -allergic effect
Active fraction
Dose - effect relationship
