米根霉HZS6生产高光学纯度L-乳酸发酵条件的研究

Study on Fermentation Conditions for High Optical Purity L-lactic Acid Produced by Rhizopus oryzae

通过研究和控制初始发酵条件,比较产物L-乳酸、D-乳酸组成变化,得出提高L-乳酸光学纯度的米根霉发酵生产条件:在pH 6.0、温度34℃、初始葡萄糖浓度100 g/L、NH4NO32 g/L、添加1.5 g/L L-乳酸的适合条件下,米根霉HZS6能够发酵生产光学纯度99%以上的L-乳酸,其转化率达到80%,终浓度为81.3 g/L。

The effect of fermentation conditions on the optical purity of L - lactic acid produced by Rhizopus oryzae HZS6 was studied. Under the suitable conditions, that is to say, pH 6. 0, 34℃, 100 g/L initial glucose, 2 g/L NH4 NO3 and 1.5 g/L supplemented L - lactic acid, Rhizopus oryzae HZS6 could produce the L-lactic acid with the optical purity of 99%, the conversion rate of 80% and the final concentration of 81.3 g/L .