实验性脑出血血肿周围组织中Rho激酶的研究

Research of the Rho kinase in perihematomal cerebral tissue after experimental intracerebral hemorrhage

目的观察实验性脑出血(ICH)血肿周围组织中Rho激酶的表达及其与神经功能缺损的关系。方法将ICH模型大鼠随机分为ICH后1d、3d、5d及7d组;在相应时间点处死大鼠前进行神经功能评分;运用免疫组化法和原位杂交法观察每组大鼠血肿周围脑组织中Rho激酶蛋白及mRNA的表达。结果ICH后1d、3d、5d及7d组神经功能评分分别为1.63、2.25、3.13及2.88;血肿周围脑组织Rho激酶蛋白阳性细胞数分别为26.63、30.75、38.88及35.00;血肿周围Rho激酶mRNA阳性细胞数分别为27.25、32.25、40.88及38.00。1d、3d、5d组间神经功能评分、Rho激酶蛋白及mRNA的表达差异有统计学意义(P<0.05～0.01),5d组与7d组间差异无统计学意义;神经功能评分与Rho激酶蛋白及mRNA的表达呈正相关(r1=0.534,r2=0.675,均P<0.01)。结论ICH后血肿周围脑组织中Rho激酶可能被激活,并参与了ICH后血肿周围脑组织的损害过程。

Objective To observe the expression of the Rho kinase in perihematomal cerebral tissue after experimental intracerebral hemorrhage （ICH） and the relationship between which and the neurological function deficit. Methods The ICH models rats were randomly divided into groups 1 d, 3 d, 5 d and 7 d after ICH. At the certain time point, the score of neurological function in each model was measured before killed. The expression of Rho kinase protein and mRNA were detected by immunohistochemistry and in situ hybridization. Results After ICH 1 d, 3 d, 5 d and 7 d groups, the scores of neurological function were 1.63, 2.25, 3.13 and 2.88,respectively; the number of Rho kinase protein positive cells in perihematomal cerebral tissue were 26.63, 30.75, 38.88 and 35.00, and the number of Rho kinase mRNA positive cells were 27.25, 32.25, 40.88 and 38.00, respectively. There were significantly differences among the groups 1 d, 3 d and 5 d in the scores of neurological function and the expression of the Rho kinase protein and mRNA（ P 〈 0. 01 -0.05 ） , but there was no significantly difference between the groups 5 d and 7 d. The expression of Rho kinase protein and mRNA were related with the neurological function deficit（ rI = 0. 534 ,r2 =0.675, all P 〈 0.01 ）. Conclusion The Rho kinase may be activated in perihematomal cerebral tissue and taken part in the process of cerebral damage after ICH.