siRNA表达载体对宫颈癌Caski细胞凋亡和增殖的影响

Influence of siRNA expression vector on cervical carcinoma Caski cell apoptosis and proliferation

目的:观察HPV16 E6 siRNA表达载体对宫颈癌Caski细胞凋亡和增殖的影响。方法:利用脂质体将HPV16 E6特异性siRNA表达载体转染Caski细胞,通过荧光定量RT-PCR检测E6 mRNA的变化,流式细胞仪检测E6蛋白和细胞凋亡率的变化,MTT法和细胞克隆法检测细胞增殖的变化。结果:HPV16 E6 siRNA表达载体可明显抑制Caski细胞E6 mRNA和E6蛋白的表达,抑制率分别为89.5%和98.1%;表达载体可明显增加细胞凋亡率,抑制细胞的增殖和克隆的形成。结论:HPV16 E6siRNA表达载体可以特异高效地抑制宫颈癌Caski细胞E6基因的表达,增加细胞的凋亡,抑制肿瘤细胞增殖和克隆的形成。

Objective To observe the effect of HPV16 E6 specific expression vector on cell proliferation and apoptosis in cervical carcinoma Caski cells.Methods HPV16 E6 siRNA expression vector and empty expression vector were transfected into Caski cells by liposome.The effects on E6 mRNA,E6 protein expression and cell apoptosis were examined respectively by real-time RT-PCR and FCM;Cell growth rate and cloning efficiency were examined by MTT and colony forming assay.Results HPV16 E6 siRNA expression vector significantly inhibited the expression levels of E6 mRNA and E6 protein,the inhibition rates being 89.5%and 98.1%respectively.Conclusion HPV16 E6 specific siRNA expression vector could specifically and efficiently inhibit the expression of E6 gene.It could induce apoptosis more effectively and inhibit cell proliferation in cervical carcinoma Caski cells.