金银花和黄芩对家兔小肠平滑肌收缩和电活动的影响

EFFECTS OF HONEYSUCKLE FLOWER AND SCUTELLARIA BAICALENSIS GEORGI ON CONSTRACTION AND ELECTRIC ACTIVITY OF RABBIT SMALL INTESTINE SMOOTH MUSCLE

目的:观察金银花和黄芩对家兔小肠平滑肌收缩和电活动的影响,并探讨其作用机制。方法:将家兔小肠离体后恒温灌流,观察金银花和黄芩对家兔小肠自发收缩和电活动的影响。结果:金银花和黄芩使家兔小肠平滑肌收缩和电活动的幅度、频率和曲线下面积明显减小,并呈剂量依赖关系。以收缩幅度为指标,用Logit法计算IC50分别为6.30g/L和1.56g/L。心得安、L-NAME和格列苯脲均可部分阻断金银花和黄芩对家兔小肠平滑肌收缩活动的抑制作用。金银花和黄芩显著抑制乙酰胆碱诱发的细胞内钙收缩幅度和细胞外钙收缩幅度。结论:金银花和黄芩显著抑制家兔小肠平滑肌的收缩和电活动。其抑制收缩活动的机制是多途径的。

Aim： To investigate the effect of Honeysuckle flower（HF） and Scutellaria Baicalensis Georgi（SBG） on contraction and electric activity of small intestine smooth muscle in rabbit and the underlying mechanisms. Methods： Using organ bath technique to observe the effect of HF and SBG on contractive and electric activity of small intestine smooth muscle in rabbit. Results： HF and SBG significantly decreased the amplitude, frequency and area under the curve of contractive, as well as electric activity in a dose-depended manner. ICs0 of the contractive amplitude was 6.30 g/L and 1.56 g/L by Logit Loglinear analysis. The inhibitive effect of HF and SBG on contractive activity could be partly decreased by β-receptor blocker Propranolol, NO synthase inhibitor L-NAME, and K^＋ channel blocker Glibenclamide. Also HF and SBG inhibited acetylcholine-induced both intracellular and extracellular calcium-depended contraction significantly. Conclusion： HF and SBG obviously inhibit the contractive and electric activity of small intestine smooth muscle of rabbit. The mechanisms are related to several pathways.