摘要
目的:探讨旋复代赭汤(IOD)拮抗顺铂(DDP)所致胃肠道毒性损伤的机理。方法:将健康杂种家兔24只随机分为3组(n=8):正常对照组、DDP组、IOD+DDP组,观察家兔体表胃电图(EGG)的改变和检测血清5-HT、5-HI-AA水平及胃窦粘膜层、十二指肠上段全层组织中5-HT、5-HIAA含量的变化,应用透射电镜观察各组超微结构改变。结果:DDP组静注DDP0.5h后,家兔EGG振幅的幅值增高、频率加快(P<0.05),同时血中5-HT、5-HIAA的浓度均升高(P<0.05),胃窦粘膜层、十二指肠组织中5-HT、5-HIAA的含量高于IOD+DDP组(P<0.05),同时电镜下可见严重损伤。而IOD能对抗DDP引起的损伤,EGG显示振幅幅值降低、频率减慢至正常水平,同时血中5-HT、5-HIAA浓度及组织中5-HT、5-HIAA的含量与DDP组对比显著降低。结论:旋复代赭汤能拮抗顺铂所致家兔EGG的改变,其机制可能与抑制胃肠道粘膜嗜铬细胞5-HT的分泌有关。
Aim： To explore the antagonism mechanism of Inulae and Ochrae Decoction on toxicity damage of gastrointestinal tissue induced by DDP （cisplatin）. Methods： Twenty-four healthy hybrid rabbits were divided into three groups in random： the control group, DDP group, Inulae and Ochrae Decoction ＋ DDP group. The change of rabbits EGG, the concentration of 5-HT,5-HIAA of serum and the content of sinus ventriculi mucosa and the upper part of duodenum tissue were examined. Ultrastructure changes were observed by transmission electron microscope （TEM）. Results： The EGG amplitude of DDP group was increased and the frequency was fast（ P 〈 0.05） after iv DDP, meanwhile the concentration of 5-HT, 5-HIAA was increased（ P 〈 0.05）, and the content of sinus ventriculi mucosa and the upper part of duodenum tissue were higher than that of Inulae and Oehrae Decoction ＋ DDP group（ P 〈 0.05）, and the change was seriously under the TEM observing; while Inulae and Ochrae Decoction could prevent the change of EGG caused by DDP, showing the amplitude decreased, and the frequency was slow, and the concentration of 5-HT, 5-HIAA of serum and the content of tissue became lower than that of DDP group. Condusion： Inulae and Ochi＇ae Decoction could antagonize the change of EGG caused by DDP, which maybe have relations with the over-releasing of gastrointestinal 5- HT.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2009年第3期377-381,共5页
Chinese Journal of Applied Physiology
基金
辽宁省教育厅高等学校科研项目(2008840)
