摘要
目的:采用RP-HPLC法同时测定广东紫珠干浸膏中熊果酸、齐墩果酸的含量。方法:采用Hypersil ODS柱(250 mm×4.6 mm),流动相为甲醇:1.0%冰醋酸水溶液(94:6),检测波长为210 nm,流速为0.5 ml/min。结果:熊果酸和齐墩果酸分别在1.010μg～5.050μg(r=0.996 4)和1.002μg～5.010μg(r=0.9983),范围内呈线性;熊果酸、齐墩果酸的平均回收率分别为99.96%(RSD=2.78%)和99.82%(RSD=2.90%)。结论:该方法简便,准确、重复性好,适用于广东紫珠干浸膏中熊果酸和齐墩果酸的质量控制。
Objective： The contents of ursolic acid and oleanolic acid in Callicarpa kwangtungensis Chun dry extract was determined by RPHPLC. Methods： The HPLC system consisted of Hypersil ODS2, mobile phase ofmethanol-1.0% acetic acid apuenous solution （96 ： 4） mixture, with detection at 210 nm, flow rate 0.5 ml/min. Result： The linear ranges of determination of ursolic acid and oleanolic acid were 1.010～5.050 μg, r=0.9964 and 1.002～5.010μg, r=0,9983, respectively, The recoveries were 99.96%（RSD 2.78%） for ursolic acid and 99.82%（RSD 2.90%） for oleanolic acid. Conclusion： The method is simple, accurate and with good reproducibility, for quality control of Callicarpa kwangtungensis Chun dry extract.
出处
《中国医药导刊》
2009年第8期1426-1427,共2页
Chinese Journal of Medicinal Guide
