摘要
目的建立阿昔洛韦分散片测定含量的HPLC方法。方法HPLC法测定条件:采用WatersSymmetryC18柱,甲醇—水(10∶90)为流动相,流速1.0ml/min,检测波长254nm。结果阿昔洛韦在4.0μg/ml～200.0μg/ml进样量范围内线性关系良好,r=0.9999(n=6),精密度为0.48%,平均回收率98.81%(RSD=0.59%,n=9)。结论本法操作简便、快速,专属性强,结果准确。
Objective To establish HPLC method to determine the content of aciclovir dispersed tablet. Methods HPLC method was established, using Waters Symmetry C18 as analysis column arid methanol, water（10：90） as mobile phase. Tile flow rate was 1.0ml/min and the detection wavelength was 254nm. Results The calibration curve was linear in the range of 4.0ug/ml-200.0ug/ml for aciclovir, r=0.9999（n=6）.The precision was 0.62%. the average revoveries were 98.81%（RSD-0.59%,n=9）. Conclusion The method apperared to be simple ,quick and specific.
出处
《当代医学》
2009年第25期140-141,共2页
Contemporary Medicine
关键词
分散片
含量测定
aciclovir
HPLC
dispersed tablet
assay
