重型再生障碍性贫血患儿外周血CD4^+CD_(25)^(high)Foxp3^+Treg细胞与CD5^+B细胞的相关性

Relationship between CD4^+CD_(25)^(high)Foxp^3+Treg Lymphocytes and CD5^+B Lymphocytes in Peripheral Blood in Children with Severe Aplastic Anemia

目的探讨重型再生障碍性贫血(SAA)患儿外周血CD4+CD25highFoxp3+Treg细胞与CD5+B细胞的变化及其相关性。方法收集36例SAA患儿(SAA组)及60例健康体检儿童(健康对照组),采用流式细胞术检测二组外周血CD4+CD25highFoxp3+Treg细胞与CD5+B细胞水平,并进行比较,同时分析SAA组此2种细胞数量的相关性;将免疫抑制治疗后有效的SAA患者划为治疗有效组,检测其CD4+CD25highFoxp3+Treg细胞与CD5+B细胞水平,并与治疗前进行比较,了解其变化趋势。结果SAA患儿外周血CD4+CD25highFoxp3+Treg细胞比例[(1.56±0.77)%]显著低于健康对照组[(3.73±0.93)%](P<0.01);CD5+B细胞比例在SAA组为(21.47±6.89)%,显著高于健康对照组[(12.21±6.18)%](P<0.01);SAA组CD4+CD25highFoxp3+Treg细胞比例与CD5+B细胞比例呈显著负相关(P<0.01)。治疗有效组CD4+CD25highFoxp3+Treg细胞比例为(2.62±0.75)%,较治疗前明显回升(P<0.05),但仍较健康对照组低(P<0.05);CD5+B细胞比例为(11.75±8.12)%,较治疗前显著下降(P<0.01)。结论CD4+CD25highFoxp3+Treg细胞与CD5+B细胞的比例改变可能参与儿童SAA的发病,CD4+CD25highFoxp3+Treg细胞的比例下降可能与CD5+B细胞的增高有关。

Objective To investigate the quantitative changes of CD4^＋ CD25 ^highFoxp3 ^＋ Treg lymphoeytes and CD5 ^＋ B lymphocytes in peripheral blood of children with severe aplastic anemia （ SAA ） and analyze the relationship between them. Methods Quantities of CD4^＋ CD25^high Foxp3^＋ Treg lymphocytes and CD5^＋ B lymphocytes in peripheral blood of 36 children with SAA （ SAA group） and 60 healthy children （healthy group） were assayed by flow cytometry. The correlation between the quantitative changes of CD4^＋ CD25 ^high Foxp3^ ＋ Treg lymphocytes and CD5^＋ B lymphocytes was analyzed. Results The quantity of CD4^ ＋ CD25^ high Foxp3^ ＋ Treg lymphocytes was significantly lower in SAA group [ （1.56 ± 0. 77 ）% ] than that in healthy group [ （3.73 ± 0. 93 ）% ] （P 〈 0.01 ）, while the quantity of CD5 ^＋B lymphocytes in SAA group was significantly higher than that in healthy group [ （ 21.47 ± 6.89 ） % vs （ 12.21 -＋ 6.18 ） %, P 〈 O. O1 ], The quantity of C D4 ~ CD25 h^gh Foxp3 ~ Treg lymphocytes showed negative correlation with CD5 ＋B lymphocytes（ P 〈 O. 01 ）. After effective immuno suppressive therapy（IST） treat- ment, the quantity of CIM ~ CD2s ~gh Foxp3 ＋ Treg lymphocytes [ （ 2.62 ＋ O. 75 ） % ] ascended significantly compared with pretherapy （ P 〈 O. 05 ） , but descended significantly compared with healthy group （ P 〈 O. 05 ）. CD5 ＊ B lymphocytes [ （ 11.75 ＋ 8.12 ） % ] descended significantly compared with pretherapy （ P 〈 O. 01 ）. Conclusions CD4 ~ CD25 high Foxp3 ＋ Treg lymphocytes in peripheral blood of children with SAA signifi- cantly decreased and CD5 ＊B lymphocytes increased,which may be related with the onset of the disease. The quantitative decrease of CD4^＋ CD25^high Foxp3^＋ Treg lymphocytes may be related with the increasing of CD5^ ＋B lymphocytes.