用酶解预处理-微波法提取积雪草总苷

Extraction of Total Asiaticoside from Centella asiatica by Enzymatic Pretreatment and Microwave Extraction

采用酶解预处理-微波法提取积雪草中的积雪草总苷。对酶解预处理温度、酶解时间、液固比、微波辐射时间等影响因素进行了单因素考察。根据考察结果选择酶解预处理温度、液固比、微波辐射时间3个因子进行二次回归正交试验设计。确定了酶解预处理-微波提取积雪草总苷的最佳工艺条件,建立了具有良好预测性能的提取模型。正交试验结果表明积雪草总苷提取的最佳工艺条件为:酶解温度45℃,液固比36,酶解时间30 min,微波辐射110 s。在此条件下积雪草总苷的得率为27.10%。该方法与传统提取法比较,具有提取速率快、目标成分得率高的特点,是较为理想的一种提取方法。

Application of enzymatic pretreatment and microwave extraction （EPME） to the extraction of asiaticoside from Centella asiatica was investigated in this paper. Temperature of enzymatic pretreatment, liquid to solid ratio, microwave radiation time were examined and selected as the factors for the quadric regression orthogonal experiments to find out the optimum operating conditions for the EPME. An extraction model with well forecast performance was also established. Results of orthogonal experiments indicated that the optimum operating conditions were as follows： temperature of enzymatic pretreatment was 45 ℃, liquid to solid ratio was 36, enzymatic time was 30 min and microwave radiation time was 110 s. Under these condition the maximum yield could reach 27.10%. Compared to the traditional extraction methods, EPME was high in extracting rate and objective constituents yield. It may be considered as an ideal means for extracting asiaticoside from Centella asiatica.