复合致病因素诱导的大鼠肝硬化并发细菌易位的研究

Study on Bacterial Translocation and Infection during the Development of Liver Cirrhosis in Rat Induced by Multiple Pathogenic Factors

目的:观察复合致病因素诱导大鼠肝硬化形成过程中肠道细菌转位以及合并感染的情况。方法:将61只大鼠随机分为模型组和正常对照组,模型组53只,正常对照组8只,观察造模过程中大鼠的感染率和死亡率。于第2、4、6、8周末取材:腹主动脉取血,测定血浆谷丙转氨酶(GPT)活力和总胆红素(TBil)水平;摘取肝脏、脾脏后称重;无菌摘取肠系膜淋巴结并进行细菌培养,观察菌落形成情况;观察大鼠合并感染情况。结果:模型复制成功,模型组大鼠合并感染率为18.6%(8/43),其中肺脏感染率为16.3%(7/43),并有细菌易位。结论:肝硬化大鼠易并发各种细菌感染,可能是导致病情加重甚至多脏器功能衰竭的重要原因。

Objective： To observe bacterial translocation and infection during the development of liver cirrhosis in rat induced by multiple pathogenic factors. Methods： Sixty - one Wistar rats were randomly divided into two groups： normal control group and hepatic cirrhosis group. Samples were collected at 2 w,4 w,6 w and 8 w respectively. GPT and TBil level in plasma were measured, and liver and spleen were weighed. Lymphoglandulae of mesenterieae was picked for detection of bacteria. Focus of infection was observed in the body. Results： The animal model of liver cirrhosis was successfully established. The total infection rate was 18.6 % （8/43） and pulmonary infection rate was 16.2% （7/43） in rats with liver cirrhosis. Meanwhile, evident bacterial translocation was found in experiment rats. Conclusion： Bacterial translocation is usually accompanied with liver cirrhosis and leads to bacterial infection, and it may accelerate primary disease and induce multipl organ dysfunctions.