TNF-α在神经病理性疼痛模型大鼠脑组织中的表达和作用

The Expression and Function of TNF-α in Brain of Rats with Neuropathic Pain

目的:通过研究保留性神经损伤(SNI)模型大鼠脑组织中TNF-α的表达情况,从而探讨TNF-α在神经病理性疼痛维持中的中枢可能机制。方法:建立SNI大鼠模型,取脑组织进行连续冰冻切片,通过免疫组织化学技术观察TNF-α在不同脑区和核团的表达情况。结果:在SNI模型大鼠的红核区域和内侧纵束间质核部位,TNF-α阳性细胞数和平均灰度值明显高于对照组,P<0.05。结论:红核以及内侧纵束间质核在神经病理性疼痛的发生和维持以及调控过程中发挥重要作用,而TNF-α是其发挥痛觉调控的主要免疫分子之一。

Objective ： To investigate the expression of TNF - α in brain of rats with spared nerve injury （SNI）, and the function of TNF- α in the maintenance of neuropathic pain. Methods ： Two weeks after SNI, immunohistochemistry was used to determine the expression of TFF - α. Results ： The level of TNF - α in Red nucleus （RN） and interstitial nucleus of the medial longitudinal fasciculus （IMLF） of SNI rats was higher than that in sham - operated rats. Conclusion： TNF - α expressed in RN and IMLF may play an important role in the development of neuropathic pain.