摘要
目的研究金葡菌耐药基因及致病因子中毒休克综合征毒素-Ⅰ(TSST-Ⅰ)基因和杀白细胞毒素(PVL)基因的分布特征。方法收集临床分离的74株金葡菌,PCR法检测毒素基因TSST-Ⅰ、PVL和mecA耐药基因。结果74株金葡菌PCR法对其行mecA基因检测,检出率为55.4%(41/74)。PVL阳性菌株的分离率为29.7%(22/74),PVL阳性的MRSA为15株(15.41,36.6%),PVL阳性的MSSA为7株(7 33,21.2%),差异无统计学意义(P>0.05)。TSST-Ⅰ基因检出率为6.8%,MSSA中未检出TSST-Ⅰ基因。结论MRSA呈多重耐药性,易造成医院内暴发流行,携带PVL和TSST-Ⅰ的金葡菌其致病力更强,应加强医院感染控制,防止其播散流行。
Objective To investigate the antibiotic-resistant gene mecA and the prevalence of Panton-Valentine leukocidin （PVL） gene, toxic shock syndrome toxin （TSST-Ⅰ ） gene in S. aureus. Methods A total of 74 S. aureus were collected from clinical specimens. The mecA, PVL and TSST-Ⅰ genes were detected by PCR. Results PVL gene was identified in 22 S. aureus isolates. The prevalence of PVL was 29.7% in S. aureus, 30.6% in MRSA and 21.2% in MSSA. The difference was not statistically significant （P〉0.05）. The prevalence of MRSA was 55.4% in 74 S. awreus . The prevalence of TSST-Ⅰ gene was 6.8%. TSST-Ⅰ gene was not detected in MSSA. Conclusions MRSA strains show highly resistant to antibiotics. PVL- and TSST-Ⅰ -positive S. aureus are more pathogenic.
出处
《中国感染与化疗杂志》
CAS
2009年第4期280-282,共3页
Chinese Journal of Infection and Chemotherapy
