临床分离金黄色葡萄球菌药物敏感性和形成生物膜的相关性分析

The correlation analysis between antibiotic susceptibility and biofilm forming ability of Staphylococcus aureus isolates

目的确定临床分离的金葡菌药物敏感性和生物膜形成能力之间的关系,为生物膜耐药机制的研究提供依据。方法采用倍比稀释法检测细菌的MIC,96孔板番红染色法检测生物膜的形成,fisher精确概率法对细菌耐药性和生物膜形成能力进行相关性分析。结果本组27株金葡菌中,除对万古霉素和米诺环素的敏感率为100%和81.5%外,对左氧氟沙星、阿米卡星、红霉素、苯唑西林、亚胺培南、克林霉素和环丙沙星均严重耐药;27株金葡菌中有17株形成了肉眼可见的生物膜,其中以X387和X409 2株最明显。结论金葡菌的耐药性与其形成生物膜的能力无相关性。

Objective The correlation between antibiotic susceptibility and biofilm forming ability was analyzed for clinical isolates of Staphylococcus aureus in order to further demonstrate the mechanism of biofilrn resistance. Methods The susceptibility of 27 S. aureus isolates to 9 antimicrobial agents were determined by agar dilution method. Screening of biofilm formation was done in 96 well plates with safranine. The correlation of antibiotic susceptibility and biofilm formation was analyzed by Fisher exact test. Results All strains were susceptible to vancomycin, and 81.5% were susceptible to rninocycline. But these strains were highly resistant to amikacin, erythromycin, ciprofloxacin, oxacillin, imipenem, clindamycin and levofloxacin. Biofilm formation was identified in 17 of the 27 strains, especially strains X387 and X409. Conclusions No correlation is found between antibiotic resistance and biofilm formation in these strains by statistical analysis.