福寿螺3个地理群体遗传多样性的AFLP分析

Analysis of genetic diversity of three geographic populations of Pomacea canaliculata by AFLP

应用AFLP分子标记技术对我国江苏苏州、福建漳州和广东珠海3个地理群体福寿螺进行了遗传多样性分析。8对选择性引物在3个群体的90个个体中,共扩增出382个位点,多态位点369个。江苏、福建和广东3个福寿螺群体的多态位点比率和Shannon’s指数分别为84.82%、85.08%、79.06%,0.4259、0.4308、0.4079;表明3个群体遗传多样性在同一水平上。不同地理来源的福寿螺个体归群分析聚成3类,与地理分布一致,表明3个地理群体间已出现遗传分化,广东群体和福建群体首先聚在一起,再与江苏群体聚类。Shannon’s指数和AMOVA分析估算均显示福寿螺的遗传变异主要来自于群体内个体间。综上所述,3个福寿螺群体具有丰富的遗传多样性而且已形成相对独立的遗传结构;并找到了3个群体间遗传特异性AFLP标记,可用于群体间分子鉴定和辅助分类。

In this study,the amplified fragment length polymorphism（AFLP） was used to analyze the genetic diversity of three populations of Pomacea canaliculata from Jiangsu,Fujian and Guangdong regions.A total of 369 polymorphic loci out of the total of 382 bands were amplified from 90 individuals.The proportion of polymorphic loci were 84.82%,85.08%,79.06% and Shannon′s Information index were 0.4259,0.4308,0.4079 in Jiangsu,Fujian and Guangdong population,respectively.These showed that there were high genetic diversity in all the three populations and the genetic diversity of three populations were in the same level.Shannon′s Information index and AMOVA analysis indicated that the genetic variation mainly came from intra-populations.The UPGMA tree based on AFLP data demonstrated that each population had independent genetic structureindividuals from the same population clustered together,Guangdong population and Fujian population first jointed,then Jiangsu population.In addition,the population specific loci were found in all the three populations which could be used as markers for inter-population molecular characterization and to assist the classification in P.canaliculata.