变应性鼻炎与Tim-3启动子区基因多态性的研究

Study on Tim- 3 promotor polymorphisms in patients with allergic rhinitis

目的探讨T细胞免疫球蛋白粘蛋白分子-3（Tim-3）启动子区-1541C〉T基因多态性与变应性鼻炎的关系。方法运用多聚酶链反应技术检测88例变应性鼻炎及102例正常人Tim-3启动子区-1541C〉T基因多态性。结果变应性鼻炎组Tim-3启动子区-1541C〉TC／C、C／T、T／T表型频率分别为：0．9733、0、0．0227，对照组分别为：0．8922、0．0882、0．1960；变应性鼻炎组Tim-3启动子区-1541C、Tim-3启动子区-1541T基因频率分别为：0．9733、0．0227，对照组分别为：0．9363、0．0637，Tim-3启动子区-1541C〉T各种基因型频率在患者组与正常对照组之间的差异无显著性（P〈0．05）。结论广东汉族人群Tim-3启动子区-1541存在单核苷酸多态性变异，Tim-3启动子区-1541C〉T基因多态性与变应性鼻炎明显相关。

Objective To investigate the relationship of Tim-3 promotor-1541 C 〉 T polymorphisms and allergic rhinitis .Methods The polymorphism of Tim-3 promotor-1541 C 〉 T polymorphisms gene was screened by PCR-RELP.Results The gene frequency of Tim-3 promotor-1541 C 〉 T polymorphisms in AR group was as followed; Tim-3 promotor -1541 C 〉 T C/C, C/T, T/T genotype frequency： 0.9733, 0, 0.0227, Tim-3 promotor-1541 C, Tim-3 promotor -1541 Genefrequency： 0.9733 , 0.0227.the gene frequency of Tim- 3 promotor-1541 C 〉 T in control group was as followed; Tim- 3 promotor -1541 C 〉 T C/C, C/T, T/T genotype frequency： 0.8922 , 0.0882, 0.196; Tim- 3 promotor-1541 C, Tim-3 promotor-1541 T Genefrequency： 0. 9363, 0.0637, There were no significant differences in the frequencies of Tim-3 promotor-1541C 〉 T gene between the patients and control group（ P〈 0. 05）. Conclusion The evident association was found between Tim- 3 promotor- 1541 C 〉 T polymorphism and allergic rhinitis.