高效液相色谱法测定人血清中司帕沙星药物浓度

Determination of the content of Sparfloxaein in human plasma by HPLC

目的建立测定人血浆中司帕沙星药物浓度的方法。方法采用二氯甲烷萃取，高效液相色谱法测定。色谱柱：GeminiC18（250mm×4．6mm，5μml流动相：0．01mol·L^-1，磷酸二氢钾-三乙胺（磷酸调PH=3．0）-甲醇（34．8：0．2：65）；流速：1．2mL·min^-1；紫外检测器：波长292nm，柱温30℃。结果司帕沙星的保留时间为9．6min，定量线性范围：0．016。2．000μg·ml^-1，方法回收率〉90％（n=5），日内、日间RSD〈10％（n=5）。结论本方法快速，定量准确，可用于血药浓度测定及人体药代动力学研究。

Objective To determine the Sparfloxacin concentration in human plasma. Methods The plasma sample was extracted by dichlormethane and determined by HPLC ,the stationary phase was Gemini C18 （250 mm × 4.6 mm, 5 μ m） , the mobile phase consisted of potassium dihydrogen phosphate-triethylamine-methanol. The flow rate was 1.2 ml.min^-1, ultraviolet detector was performed with wavelength detector at 292 nm. Results The retention time of Sparfloxacin was about 9.6 min, and the linear range of quantity was 0.016- 2.000 μ g. ml^-1. The recoveries of method were more than 90%（n=5）, RSD 〈 10 % （n=5）. Conclusions This method is rapid and accurate, it can be used for the content determination of the plasma Sparfloxacin and the study of pharmacokinetics.