色氨酸羟化酶2基因多态性与强迫症的关联研究

Association between tryptophan hydroxylase-2 gene and obsessive-compulsive disorder

目的探索汉族人群中具有中枢特异性的色氨酸羟化酶2(TPH2)基因多态性与强迫症的关系。方法选取TPH2基因转录区的单核苷酸多态rs4570625,采用TaqM an探针SNP基因分型技术测定137例强迫症患者和190名健康人的多态分布。结果强迫症组色氨酸羟化酶(TPH)-2基因rs4570625G/T多态基因型及等位基因频数多态分布与对照组间有统计学差异(χ2=9.972,P<0.01;χ2=8.417,P<0.01);GG基因型和G等位基因与强迫症之间存在显著正关联[比数比(OR)值分别为2.239和1.587]。早发型患者组该多态基因型及等位基因频数多态分布与对照组间有显著差异(χ2=9.202,P<0.05;χ2=8.833,P<0.01);GG基因型和G等位基因与早发型强迫症之间存在显著正关联[OR值分别为2.514和1.886],晚发型患者组与对照组间无统计学差异。结论在汉族人群中色氨酸羟化酶TPH2基因rs4570625G/T多态可能与强迫症存在遗传关联,GG基因型和等位基因G可能主要是早发型强迫症的风险因子。

Objective： To explore the association between the brain - specific tryptophan hydroxylase - 2 （TPH2） gene polymorphism and obsessive- compulsive disorder（OCD） in the Han nationality. Methods： The single nucleotide polymorphism （SNPs） of TPH2 in the transcriptional control region, rs4570625, was analyzed by TaqMan SNP Genotyping Assay in 137 OCD patients and 190 healthy controls. Results ：There were significant differences between patients and controls on genotype and allele frequencies of TPH2 SNP rs4570625 （X^2 =9.972 ,P 〈0.01 ;X^2 = 8. 417,P 〈0.01 ）. The GG genotype and G allele were positively associated with OCD （OR values were 2. 239 and 1. 587, respectively）. There were significant differences between early - onset patients and controls on genotype and allele frequencies of TPH2 SNP rs4570625 （X^2 = 9. 202, P 〈 0.05 ; X^2 = 8. 833 ,P 〈0.01 ）. The GG genotype and G allele were positively associated with OCD （OR were 2. 514 and 1. 886, respectively）. No significant difference was found between late -onset patients and controls（P 〉 0.05 ）. Conclusion：The results suggest that the TPH2 SNP rs4570625 may be associated with OCD in the Han nationality. GG genotype and G allele may be the risk factors of early - onset OCD.