磷酸三邻甲苯酯对母鸡神经组织线粒体膜通透性的影响

Effects of Tri-ortho-cresyl Phosphate on Mitochondrial Membrane Permeability in Hen's Nerve Tissue

目的研究磷酸三邻甲苯酯(tri-ortho-cresyl phosphate,TOCP)对母鸡神经组织线粒体膜通透性的影响,探讨有机磷诱导迟发性神经毒性的机制。方法成年罗曼母鸡随机分为4组,每组24只,TOCP溶解于玉米油中,染毒组分别按185、375、750mg/kg经灌胃一次性染毒,灌胃量为0.65ml/kg,对照组给予等体积玉米油。分别于染毒后第1、5、15、21天处死动物,迅速剥离母鸡大脑、小脑、脊髓组织,分离其线粒体,测定线粒体膜通透性和跨膜电位的改变。结果染毒组与对照组相比,大脑中线粒体膜通透性改变不明显;小脑375、750mg/kg组在染毒第1、5天时通透性有明显增加(P<0.05);染毒第1天时3个染毒组脊髓中线粒体膜通透性均明显增加(P<0.05),染毒第5、15天时375、750mg/kg组膜通透性明显增加(P<0.05)。各剂量组间及各时间点之间跨膜电位均未观察到明显改变。结论TOCP能明显增加母鸡小脑及脊髓中线粒体膜通透性。

Objective To study the effects of tri-ortho-cresyl phosphate （TOCP） on mitochondrial membrane permeability in hen＇s nerve tissues and investigate the mechanism of the organophosphorns ester-induced delayed neurotoxicity （OPIDN）. Methods Adult Roman hens were randomly divided into four groups, including three treated groups and one control group （24 in each group）. The hens in the experimental groups were treated with TOCP by gavage at the single dosages of 185, 375 and 750 mg/kg respectively. TOCP was dissolved in corn oil and administered at 0.65 ml/kg. The control hens received an equivalent volume of corn oil by garage. The hens were sacrificed on the 1st, 5th, 15th and 21st day after treatment and the cerebrum, cerebellum, spinal cord were dissected and homogenized in ice bath. The mitochondria in these nerve tissues were extracted to determine the changes of the membrane permeability and membrane potential. Results Compared with the control group, no significant increase of the mitochondrial membrane permeability in the cerebrum was observed in treated groups. In the cerebellum, the membrane permeability in the 185 mg/kg group had no significant changes, while in the 375 and 750 mg/kg groups it increased significantly on the 1st and 5th day after TOCP treatment （P〈0.05）. In spinal cord, the membrane permeability in the three treated groups all increased significantly on the 1st day （P〈0.05）, and on the 5th and 15th day, the membrane permeability in the 375 and 750 mg/kg groups increased significantly （P〈0.05）. No significant difference was seen in the membrane permeability among the three treated groups as time went on, also there was no significant difference in the membrane potential among the three treated groups as time went on. Conclusion TOCP can significantly increase the permeability of the mitochondrial membrane in hen＇s cerebellum and spinal cord and that might be one of the mechanisms of OPIDN development.