摘要
目的研究化妆品中污染的微生物对常用防腐剂抵抗性,为化妆品的防腐研究提供依据。方法参照《消毒技术规范》(2002)利用最小抑菌浓度(MIC)的方法测定化妆品中分离菌株(泛菌属,日沟维肠杆菌,浅黄假单胞菌,科氏葡萄球菌,木霉菌,拟青霉菌,毛霉菌,青霉菌等)对常用防腐剂对羟基苯甲酸酯类(尼波金酯类)、1,2-溴-2-硝基-1,3-丙二醇(布罗波尔)、咪唑烷基脲、凯松的抵抗性。结果部分分离菌株对常用防腐剂的抵抗性较高,日沟维肠杆菌对对羟基苯甲酸甲酯的MIC为10mg/ml,拟青霉对对羟基苯甲酸丙酯的MIC为10mg/ml,分别为以上防腐剂限用剂量的2.5倍;泛菌属、浅黄假单胞菌和木霉菌对咪唑烷基脲的MIC远高出其他标准菌株。结论部分分离菌株对常用防腐剂的抵抗性较高,提示在化妆品中污染的微生物可能对常用防腐剂产生了耐受性,而且可能是化妆品微生物污染的原因之一,在防腐剂的选用过程中应引起重视。
Objective To research the resistibility of microbes contaminated in cosmetics to preservatives in common use in cosmetics. Methods The minimum inhibitory concentration （MIC） method was used according to Disinfection Technical Guideline. Results The resuls showed that some of the isolated microbes had higher resistance for one or more of the commonly used preservatives. The MIC of enterobacter gergoviae for methyl paraben was 10 mg/ml, the MIC of paecilomyces for propyl paraben was 10 mg/ml, it was 2.5 times greater than the limited dose. The MIC of Pantoea, P.luteola and tr/choderma for imidazolidinyl urea were higher than other standard microbe strains. Conclusion The microbes contaminated in cosmetics can develop tolerance to the preservatives in common use in cosmetics, maybe that is one of the causes for cosmetics contamination.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2009年第8期694-695,共2页
Journal of Environment and Health
关键词
化妆品
防腐剂
耐受性
Cosmetics
Preservatives
Resistibility
