肺癌组织中OATP-B和OATP-D的mRNA及其蛋白表达和临床意义

Expression and clinical significance of organic anion transporting polypeptide mRNA and protein in lung cancers

目的探讨人类有机阴离子转运多肽(OATP)家族中的OATP-B和OATP-D的mRNA及其蛋白表达和肺癌的关系。方法采用实时荧光定量PCR(FQ-PCR)法检测江苏省淮安市第一人民医院和青岛市立医院2004—2006年收集的40例肺癌组织和10例正常肺组织中OATP-B和OATP-D的mRNA的含量;免疫组织化学法检测40例肺癌组织和20例癌旁组织中OATP表达。结果OATP-B和OATP-D的mRNA及蛋白表达在肺癌组织中表达均上调,与正常肺组织比较,差异有统计学意义(P<0.05)。各种病理类型之间比较,差异无统计学意义(P>0.05)。有淋巴结转移的肺癌组织中OATP-B和OATP-D的mRNA及蛋白表达上调与无淋巴结转移的肺癌组织比较,差异有统计学意义(P<0.05)。OATP-B和OATP-D的mRNA及蛋白表达与肿瘤直径无关。结论各组织类型肺癌与OATP-B和OATP-D的表达相关,与淋巴结转移相关。OATP-B和OATP-D的表达异常与肿瘤的大小、性别及年龄无关。

Objective To study the expression and clinical significance of organic anion transporting polypeptide（ OATP- B and OATP-D）mRNA and protein in lung cancers. Methods . The expressions of OATP-B and OATP-D mRNA were detected in 40 cases of lung cancer and 10 cases normal lung tissues by SYBRGreen Quantitative Real-time PCR,as well as the expressions of OATP-B and OATP-D were discovered by Immunohistochemistry analysis. Results The expressions of OATP-B and OATP-D mRNA were all significantly up-regulated in different pathological category lung neoplasms compared to normal lung tissues. There was also a significant difference in the expressions of OATP-B and OATP-D mRNA between lymph metastasis lung neoplasms and those without lymph metastasis （ P 〈 0.05 ）. Immunohistochemistry analysis showed a higher expression of OATP-B and OATP-D protein in lung cancers than normal lung tissues, as well as in lung cancers with lymph metastasis compared to those without lymph metastasis （ P 〈 0. 05 ）. Conclusion The expression of OATP-B and OATP-D are associated with lung cancer and with lymph metastasis respectively.