E-cadherin在结直肠癌中的表达及其与侵袭转移的关系

Expresssion of E-cadherin in colorectal carcinoma and the invasion and metastasis

目的:探讨E-cadherin在结直肠癌(colorectal carcinoma,CRC)中的表达及其与侵袭转移的关系。方法:采用免疫组织化学SABC法及原位杂交技术检测E-cadherin mRNA和蛋白在大肠正常黏膜、大肠腺瘤和CRC组织中的表达。结果:E-cadherin的mRNA和蛋白在CRC中的阳性率显著低于正常黏膜组织和腺瘤(P<0.01);随着CRC分化的降低和Dukes分期的增加,E-cadherin表达阳性率降低;E-cadherin的阳性率在有淋巴结和远处器官转移的CRC中均显著低于未转移组(P<0.01,P<0.05)。结论:E-cadherin表达减少或缺失在CRC的侵袭转移过程中发挥重要的作用。

Objective To investigate the relation between the invasion and metastasis of colorectal carcinoma and the expression of E-cadherin. Methods The immunohistochemistry method and the in situ hybridization method were used to detect the expression of E-cadherin in normal colorectal mucosa,colorectal adenomas, and colorectal carcinomas tissues. Results The positive rate of mRNA and protein of E-cadherin in colorectal carcinoma were lower than that of normal colorectal mucosa and adenomas tissues （P 〈0.01 ） ; the positive rate of E-cadherin were decreased concomitantly with decrease in differentiation of CRC and the increase in Dukes stages; the positive rate of E-cadherin in colorectal carcinoma of the metastasis of lymph node and distant organ was lower than that of the negative ones（ P 〈 0.01,P 〈 0.05）. Conclusion The decrease and absence of E-cadherin play an important role in the invasion and metastasis of colorectal carcinoma.