HPLC法测定余甘子喉片中没食子酸的含量

Determination of Gallic Acid in Fructus Phyllanthi Throat Tablets by HPLC

目的:建立测定余甘子喉片中没食子酸含量的HPLC方法。方法:色谱柱:Agilent ZORBAX Eclipse XDB-C_(18)(150mm×4.6mm,5μm)柱;流动相:甲醇-水-磷酸(5:95:0.05);流速:1.0ml·min^(-1);检测波长:280nm;柱温:30℃,外标定量法。结果:没食子酸在0.09～0.56 mg·ml^(-1)呈良好的线性关系(r=0.999 9),平均回收率为99.8%(n=6),RSD=0.27%。结论:本法操作简单,灵敏,准确,可以用于余甘子喉片的质量控制。

Objective： To establish an HPLC method for determination the content of gallic acid in fructus phyllanthi throat tablets. Method： The Agilent ZORBAX Eclipse XDB-C18 （150mm ×4.6mm,5μm）column was used,the mobile phase was methanol-water-phosphate（5：95： 0.05 ）, the flow rate was 1.0ml·min^-1, and the detection wavelength was at 280 nm, column temperature was 30℃, and outside demarcates was used as the mensuration. Result： The linearity of gallic acid was within the range of 0.09 - 0.56mg · ml^ - 1 （ r = 0.999 9）, and the average recovery was 99.8% （ n = 6） （ RSD% = 0.27% ）. Conclusion ： This method is simple, sensitive and accurate, and available for control the quality of fructus phyllanthi throat tablets.