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糖基化终产物对人肾系膜细胞趋化因子RANTES表达的影响 被引量：3

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摘要 目的探讨糖基化终产物(AGEs)对人肾系膜细胞(HRMC)趋化因子RANTES表达的影响。方法常规方法制备糖基化终产物(AGEs-BSA),干预体外培养的HRMC,收集培养上清和细胞,ELISA法检测上清中RANTES浓度,实时定量RT-PCR检测RANTES mRNA表达水平,Western blot检测RANTES蛋白表达,分别设立同浓度的BSA组及空白对照组作为对照。结果AGEs-BSA干预组HRMC RANTES mRNA和蛋白表达水平及上清中RANTES浓度明显高于对照组,并存在浓度和时间依赖效应。结论AGEs-BSA上调HRMC RANTES表达和分泌,可能参与糖尿病肾病的发生。
作者 马丽 孙子林 王少华
机构地区 东南大学附属中大医院内分泌科 武警南京消防医院内科
出处 《现代医学》 2009年第3期209-211,共3页 Modern Medical Journal
关键词 糖基化终产物 人肾系膜细胞 正常T细胞表达分泌的调节趋化蛋白
分类号 R-33 [医药卫生] R587.1 [医药卫生—内分泌]
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参考文献11

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二级参考文献34

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共引文献14

同被引文献39

引证文献3

二级引证文献7

现代医学
2009年 第3期

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